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mouse antibovine wc1  (Bio-Rad)


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    Bio-Rad mouse antibovine wc1
    FIG. 1. Leukocytes in efferent lymph and peripheral blood of can- nulated, uninfected sheep 455. Cell counts are plotted in thousands per l as a function of time after cannulation for efferent lymph cells (A) and leukocytes (B) (closed symbols) and mononuclear cells (open symbols) from peripheral blood. Percentages of live-gated, mononu- clear cells staining for the surface markers IgM, CD4, CD8, <t>WC1,</t> and CD14 are plotted for efferent lymph (C) and peripheral blood (D).
    Mouse Antibovine Wc1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antibovine+wc1/Mouse+anti+Bovine+WC1/10__1128_slash_jvi__00529___06-119-49-58
    Average 94 stars, based on 41 article reviews
    mouse antibovine wc1 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node"

    Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node

    Journal: Journal of Virology

    doi: 10.1128/jvi.00529-06

    FIG. 1. Leukocytes in efferent lymph and peripheral blood of can- nulated, uninfected sheep 455. Cell counts are plotted in thousands per l as a function of time after cannulation for efferent lymph cells (A) and leukocytes (B) (closed symbols) and mononuclear cells (open symbols) from peripheral blood. Percentages of live-gated, mononu- clear cells staining for the surface markers IgM, CD4, CD8, WC1, and CD14 are plotted for efferent lymph (C) and peripheral blood (D).
    Figure Legend Snippet: FIG. 1. Leukocytes in efferent lymph and peripheral blood of can- nulated, uninfected sheep 455. Cell counts are plotted in thousands per l as a function of time after cannulation for efferent lymph cells (A) and leukocytes (B) (closed symbols) and mononuclear cells (open symbols) from peripheral blood. Percentages of live-gated, mononu- clear cells staining for the surface markers IgM, CD4, CD8, WC1, and CD14 are plotted for efferent lymph (C) and peripheral blood (D).

    Techniques Used: Staining

    FIG. 5. Cell lineages and blasts among efferent lymph cells of mock-infected and infected sheep. (A) Percentages of live cell labeling on the surface for IgM, CD4, CD8, WC1, and CD14 are plotted for each animal relative to the day of injection with allogeneic cells. Horizontal reference lines represent the average percentage for a given lineage as measured in each animal before injection of allogeneic cells. (B) Reciprocal oscillation of IgM and CD8 lymph cells in infected, cannulated sheep. (C) Percentages of blasts among lymph cells of different lineages. Blasts were quantified as percentages of lymph cells staining for the indicated surface marker by gating on cells with high forward and side scatter (infected animals, closed symbols; mock-infected animals, open symbols). Averages for infected (solid lines) and mock-infected (dotted lines) animals were calculated using measurements made either on the same day or on adjacent days after injection of allogeneic cells.
    Figure Legend Snippet: FIG. 5. Cell lineages and blasts among efferent lymph cells of mock-infected and infected sheep. (A) Percentages of live cell labeling on the surface for IgM, CD4, CD8, WC1, and CD14 are plotted for each animal relative to the day of injection with allogeneic cells. Horizontal reference lines represent the average percentage for a given lineage as measured in each animal before injection of allogeneic cells. (B) Reciprocal oscillation of IgM and CD8 lymph cells in infected, cannulated sheep. (C) Percentages of blasts among lymph cells of different lineages. Blasts were quantified as percentages of lymph cells staining for the indicated surface marker by gating on cells with high forward and side scatter (infected animals, closed symbols; mock-infected animals, open symbols). Averages for infected (solid lines) and mock-infected (dotted lines) animals were calculated using measurements made either on the same day or on adjacent days after injection of allogeneic cells.

    Techniques Used: Infection, Labeling, Injection, Staining, Marker

    FIG. 8. Percentages of BrdU cells present among efferent lymph cells and PBMCs after intravenous pulses of BrdU. The percentage of BrdU-stained cells, as determined by flow cytometry, is plotted for the total live-cell population as well as within IgM, CD4, CD8, and WC1
    Figure Legend Snippet: FIG. 8. Percentages of BrdU cells present among efferent lymph cells and PBMCs after intravenous pulses of BrdU. The percentage of BrdU-stained cells, as determined by flow cytometry, is plotted for the total live-cell population as well as within IgM, CD4, CD8, and WC1

    Techniques Used: Staining, Cytometry

    Related Articles

    Affinity Purification:

    Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node
    Article Snippet: .. Primary antibodies to surface proteins were as follows: affinity-purified rabbit-anti-sheep immunoglobulin (Kirkegaard & Perry Laboratories) used at 1:200; mouse-anti-bovine CD4 (clone GC1A, IgG2a isotype; cross-reactive with sheep; Veterinary Medical Research and Development, Inc.) used at 1:50; mouse-anti-bovine CD8 (clone CC63, IgG2a isotype; cross-reactive with sheep; Serotec) used at 1:50; mouse-antibovine WC1 (clone CC15, IgG2a isotype; cross-reactive with sheep; Serotec) used at 1:50 to detect a major subpopulation of T cells (41); and affinitypurified mouse-anti-sheep CD14 (clone VPM65, IgG1 isotype; Serotec) (16) used at 1:50. .. For double labeling, mouse-anti-human CD14 (clone TÜK4, IgG2a isotype; cross-reactive with sheep; Serotec) conjugated to phycoerythrin-Cy5 was used at 1:50.



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    mouse antibovine wc1  (Bio-Rad)
    94
    Bio-Rad mouse antibovine wc1
    FIG. 1. Leukocytes in efferent lymph and peripheral blood of can- nulated, uninfected sheep 455. Cell counts are plotted in thousands per l as a function of time after cannulation for efferent lymph cells (A) and leukocytes (B) (closed symbols) and mononuclear cells (open symbols) from peripheral blood. Percentages of live-gated, mononu- clear cells staining for the surface markers IgM, CD4, CD8, <t>WC1,</t> and CD14 are plotted for efferent lymph (C) and peripheral blood (D).
    Mouse Antibovine Wc1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antibovine+wc1/Mouse+anti+Bovine+WC1/10__1128_slash_jvi__00529___06-119-49-58
    Average 94 stars, based on 1 article reviews
    mouse antibovine wc1 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

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    FIG. 1. Leukocytes in efferent lymph and peripheral blood of can- nulated, uninfected sheep 455. Cell counts are plotted in thousands per l as a function of time after cannulation for efferent lymph cells (A) and leukocytes (B) (closed symbols) and mononuclear cells (open symbols) from peripheral blood. Percentages of live-gated, mononu- clear cells staining for the surface markers IgM, CD4, CD8, WC1, and CD14 are plotted for efferent lymph (C) and peripheral blood (D).

    Journal: Journal of Virology

    Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node

    doi: 10.1128/jvi.00529-06

    Figure Lengend Snippet: FIG. 1. Leukocytes in efferent lymph and peripheral blood of can- nulated, uninfected sheep 455. Cell counts are plotted in thousands per l as a function of time after cannulation for efferent lymph cells (A) and leukocytes (B) (closed symbols) and mononuclear cells (open symbols) from peripheral blood. Percentages of live-gated, mononu- clear cells staining for the surface markers IgM, CD4, CD8, WC1, and CD14 are plotted for efferent lymph (C) and peripheral blood (D).

    Article Snippet: Primary antibodies to surface proteins were as follows: affinity-purified rabbit-anti-sheep immunoglobulin (Kirkegaard & Perry Laboratories) used at 1:200; mouse-anti-bovine CD4 (clone GC1A, IgG2a isotype; cross-reactive with sheep; Veterinary Medical Research and Development, Inc.) used at 1:50; mouse-anti-bovine CD8 (clone CC63, IgG2a isotype; cross-reactive with sheep; Serotec) used at 1:50; mouse-antibovine WC1 (clone CC15, IgG2a isotype; cross-reactive with sheep; Serotec) used at 1:50 to detect a major subpopulation of T cells (41); and affinitypurified mouse-anti-sheep CD14 (clone VPM65, IgG1 isotype; Serotec) (16) used at 1:50.

    Techniques: Staining

    FIG. 5. Cell lineages and blasts among efferent lymph cells of mock-infected and infected sheep. (A) Percentages of live cell labeling on the surface for IgM, CD4, CD8, WC1, and CD14 are plotted for each animal relative to the day of injection with allogeneic cells. Horizontal reference lines represent the average percentage for a given lineage as measured in each animal before injection of allogeneic cells. (B) Reciprocal oscillation of IgM and CD8 lymph cells in infected, cannulated sheep. (C) Percentages of blasts among lymph cells of different lineages. Blasts were quantified as percentages of lymph cells staining for the indicated surface marker by gating on cells with high forward and side scatter (infected animals, closed symbols; mock-infected animals, open symbols). Averages for infected (solid lines) and mock-infected (dotted lines) animals were calculated using measurements made either on the same day or on adjacent days after injection of allogeneic cells.

    Journal: Journal of Virology

    Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node

    doi: 10.1128/jvi.00529-06

    Figure Lengend Snippet: FIG. 5. Cell lineages and blasts among efferent lymph cells of mock-infected and infected sheep. (A) Percentages of live cell labeling on the surface for IgM, CD4, CD8, WC1, and CD14 are plotted for each animal relative to the day of injection with allogeneic cells. Horizontal reference lines represent the average percentage for a given lineage as measured in each animal before injection of allogeneic cells. (B) Reciprocal oscillation of IgM and CD8 lymph cells in infected, cannulated sheep. (C) Percentages of blasts among lymph cells of different lineages. Blasts were quantified as percentages of lymph cells staining for the indicated surface marker by gating on cells with high forward and side scatter (infected animals, closed symbols; mock-infected animals, open symbols). Averages for infected (solid lines) and mock-infected (dotted lines) animals were calculated using measurements made either on the same day or on adjacent days after injection of allogeneic cells.

    Article Snippet: Primary antibodies to surface proteins were as follows: affinity-purified rabbit-anti-sheep immunoglobulin (Kirkegaard & Perry Laboratories) used at 1:200; mouse-anti-bovine CD4 (clone GC1A, IgG2a isotype; cross-reactive with sheep; Veterinary Medical Research and Development, Inc.) used at 1:50; mouse-anti-bovine CD8 (clone CC63, IgG2a isotype; cross-reactive with sheep; Serotec) used at 1:50; mouse-antibovine WC1 (clone CC15, IgG2a isotype; cross-reactive with sheep; Serotec) used at 1:50 to detect a major subpopulation of T cells (41); and affinitypurified mouse-anti-sheep CD14 (clone VPM65, IgG1 isotype; Serotec) (16) used at 1:50.

    Techniques: Infection, Labeling, Injection, Staining, Marker

    FIG. 8. Percentages of BrdU cells present among efferent lymph cells and PBMCs after intravenous pulses of BrdU. The percentage of BrdU-stained cells, as determined by flow cytometry, is plotted for the total live-cell population as well as within IgM, CD4, CD8, and WC1

    Journal: Journal of Virology

    Article Title: Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node

    doi: 10.1128/jvi.00529-06

    Figure Lengend Snippet: FIG. 8. Percentages of BrdU cells present among efferent lymph cells and PBMCs after intravenous pulses of BrdU. The percentage of BrdU-stained cells, as determined by flow cytometry, is plotted for the total live-cell population as well as within IgM, CD4, CD8, and WC1

    Article Snippet: Primary antibodies to surface proteins were as follows: affinity-purified rabbit-anti-sheep immunoglobulin (Kirkegaard & Perry Laboratories) used at 1:200; mouse-anti-bovine CD4 (clone GC1A, IgG2a isotype; cross-reactive with sheep; Veterinary Medical Research and Development, Inc.) used at 1:50; mouse-anti-bovine CD8 (clone CC63, IgG2a isotype; cross-reactive with sheep; Serotec) used at 1:50; mouse-antibovine WC1 (clone CC15, IgG2a isotype; cross-reactive with sheep; Serotec) used at 1:50 to detect a major subpopulation of T cells (41); and affinitypurified mouse-anti-sheep CD14 (clone VPM65, IgG1 isotype; Serotec) (16) used at 1:50.

    Techniques: Staining, Cytometry